| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Nigerium massiliense | TCATCCAGGTCGGCTTGTG | TCGATGAACGTCGGCATCC | 59.70 | 60.23 | 223 | 162 |
100.00%
|
100.00%
|
| Primer 2 | Nigerium massiliense | AGATGATGCACTCCCGTGG | ACTGCTCGTCATCGCTTGG | 59.48 | 60.45 | 158 | 156 |
100.00%
|
100.00%
|
| Primer 3 | Nigerium massiliense | ATGTCCGAATCCCGTCTGC | TCGTCGAGAAGCTGGTTGAG | 59.86 | 59.76 | 170 | 148 |
100.00%
|
100.00%
|
| Primer 4 | Nigerium massiliense | ACCATCGAGAAGGTGCAGG | TGCGCTTGATCACCGTGT | 59.40 | 59.97 | 157 | 148 |
100.00%
|
100.00%
|
| Primer 5 | Nigerium massiliense | ATCGAGAAGGTGCAGGACC | TGCGCTTGATCACCGTGT | 59.10 | 59.97 | 154 | 148 |
100.00%
|
100.00%
|
| Primer 6 | Nigerium massiliense | TTGGTTCGCGTTCGGAGT | CGAGACACGCCAGAACAAC | 59.58 | 59.15 | 298 | 143 |
100.00%
|
100.00%
|
| Primer 7 | Nigerium massiliense | TTGGTTCGCGTTCGGAGT | GACACGCCAGAACAACCAG | 59.58 | 59.06 | 295 | 143 |
100.00%
|
100.00%
|
| Primer 8 | Nigerium massiliense | ACCATCCACTCCCATCTGC | AGGTTGCGCTGGTCGAT | 59.08 | 58.93 | 290 | 141 |
100.00%
|
100.00%
|
| Primer 9 | Nigerium massiliense | CCATCGAGAAGGTGCAGGA | TGCGCTTGATCACCGTGT | 59.10 | 59.97 | 156 | 140 |
100.00%
|
100.00%
|
| Primer 10 | Nigerium massiliense | AACTCGCACTGATGGAGCC | CCAGGGCTGACAAGAGCAT | 60.08 | 59.70 | 252 | 138 |
100.00%
|
100.00%
|